Fluorescence Immunoassay Test Kits

iQuant Analyser

iQuant is an immunofluorescence assay reader. Quantitative measurements of intended analytes in human blood/serum/plasma is performed by reading Quanti lateral immunofluorescence assay (FIA) cartridge device. The iQuant instrument scans this device, detects the relevant signal using fluorescence detection and computes the final result of the test by applying a proprietary calibration method.

Fluorescence Immunoassay Test Kits Range

Vitamin D Quanti Card

INTENDED USE
Vitamin D Quanti Card is a sensitive immunoassay for the quantitative determination of Vitamin D (Total) in human serum with device reader.

PRINCIPLE (ANTIGEN-ANTIBODY REACTION)
Vitamin D Quanti Card is based on principle of competitive immunoassay. The vitamin D from the sample is first released/extracted and stabilized. The assay is based on competition between extracted vitamin D from test sample and vitamin D biotin conjugate for the limited no of binding sites of specfific antibodies fluorochrome conjugate. As a result higher concentration of vitamin D produces a lower fluorescence signal and vice versa. The signal is interpreted and displayed on device reader in form of results.

HbA1c Quanti Card

INTENDED USE
HbA1c Quanti Card is a rapid and sensitive immunoassay for the quantitative determination of HbA1c (Glycated hemoglobin) in human whole blood with i-Quant Analyzer. The test is used for routine monitoring of long term glycemic status in patient with diabetes mellitus.

PRINCIPLE (ANTIGEN-ANTIBODY REACTION)
HbA1c Quanti Card is based on fluorescence immunoassay technology. The test uses anti-HbA1c antibody and anti-hemoglobin antibody immobilized separately on a nitrocellulose strip. Whole blood sample is added to the mixture of Detection Buffer and Assay Buffer, resulting in hemolysis of red blood cells. The mixture containing HbA1c and Hb from the hemolyzed red blood cells and fluorescence labelled HbA1c and Hb from Detection Buffer, is added to the sample well of the cartridge. The mixture migrates through the nitrocellulose strip by capillary action. When the complex meets the line of the corresponding immobilized antibody on nitrocellulose strip, the fluorescence signal is produced from immuno complex. The signal is interpreted and the result is displayed on i-Quant Analyzer in terms of percentage.

DENGUE NS1 Ag Quanti Card

INTENDED USE
Dengue NS1 Ag Quanti Card is a sensitive immuno- chromatographic test for the qualitative detection of Dengue NS1 Antigen in human Whole Blood/ Serum/ Plasma with iQuant Analyzer . This test is for in vitro diagnostic use only and is intended as an aid in the earlier diagnosis of dengue infection.

PRINCIPLE (ANTIGEN-ANTIBODY REACTION)
Dengue NS1 Ag Quanti Card is a fluorescence immunoassay based on sandwich principle. The test area is coated with specific anti-dengue NS1 Ag antibodies. When a sample is added to the cartridge, Dengue NS1 antigen if present will form a complex with another specific NS1 antibodies conjugate to fluorochorme. On addition of Assay Buffer, this complex migrates along the nitrocellulose membrane to the test region and forms an antibody-antigen-antibody fluorescence immunocomplex. The result will be displayed by i-Quant Analyzer.

T3 Quanti Card

INTENDED USE
T3 Quanti Card is a sensitive immunoassay for the quantitative determination of Triiodothyronin (T3)in human serum/ plasma with iQuant Analyzer.
PRINCIPLE (ANTIGEN-ANTIBODY REACTION)
T3 Quanti Card is based on principle of competitive immunoassay. The T3 from the sample is first released/extracted. Extracted T3 from test sample and T3 biotin conjugate competes for the limited number of binding sites of specific antibodies fluorochrome conjugate. As a result higher concentration of T3 produces a lower fluorescence signal and vice versa. The signal is interpreted and displayed on iQuant Analyzer in form of results.

T4 Quanti Card

INTENDED USE
T4 Quanti Card is a sensitive immunoassay for the quantitative determination of T4 (Thyroxine) in human serum/ plasma with iQuant Analyzer.
PRINCIPLE (ANTIGEN-ANTIBODY REACTION)
T4 Quanti Card is based on principle of competitive immunoassay. The T4 from the sample is first released/extracted. Extracted T4 from test sample and T4 biotin conjugate competes for the limited number of binding sites of specific antibodies fluorochrome conjugate. As a result higher concentration of T4 produces a lower fluorescence signal and vice versa. The signal is interpreted and displayed on iQuant Analyzer in form of results.

TSH Quanti Card

INTENDED USE
TSH Quanti Card is a rapid and sensitive immunoassay for the quantitative determination of Thyroid Stimulating Hormone (TSH) in human serum/ plasma with i-Quant Analyzer.

PRINCIPLE (ANTIGEN-ANTIBODY REACTION)
TSH Quanti Card is based on principle of fluorescence immunoassay technology. The test uses anti TSH antibody immobilized on a nitrocellulose strip. Sample is mixed with Detection Buffer. The TSH from the sample and fluorescence labeled anti TSH antibody from Detection Buffer forms a complex. The complex migrates through the nitrocellulose strip by capillary action. When the complex meets the line of the corresponding specific antibody immobilized on nitrocellulose membrane, the fluorescence signal is produced. This signal is interpreted and the result is displayed on iQuant Analyzer in form of results.