Blood Grouping Sera

ANTI A-MONOCLONAL

INTENDED USE

Anti-A Monoclonal Antibodies are in-vitro culture supernatant of hybrids obtained by cellular fusion (Hybridoma Technology). It is a monoclonal antibody for ABO grouping. The reagent is for in-vitro use only.

The ABO Grouping is defined both by the presence of ‘A’ and/or B’ antigens on the surface of the red blood cells and by the simultaneous presence of anti-A and/or anti-B antibodies in the serum. An individual has in his serum the antibodies corresponding to the antigens which are not on his red blood cells. It is thus necessary to identify the erythrocyte antigens by the known ANTI- A, ANTI- B and ANTI- AB monoclonal antibodies(forward typing) and then confirm the results by verifying the presence of corresponding antibodies in the blood serum to be tested with control cells:’A1,‘A2, ‘B’ and ‘O’ (Reverse typing). Human red blood cells possessing ‘A’ and /or ‘B’ antigen will agglutinate in the presence of antibody directed towards the antigen. For example agglutination of RBCs with Anti –A monoclonal antibodies indicates the presence of ‘A’ Antigen on the red blood cells.

The Antibodies are stable at 2-8°C for 18 Months.

ORDERING INFORMATION

PRODUCT NAME	PACK SIZE	CATALOG NO.
ANTI A-MONOCLONAL ANTIBODIES	10 ml	BR230010

PERFORMANCE CHARACTERISTICS

Cells	Avidity	Intensity	Titre
A1	2-4 seconds	++++	≥256
A2	3-5 seconds	++	≥64

ANTI B MONOCLONAL ANTIBODIES

INTENDED USE

Anti-B Monoclonal Antibodies is an in-vitro culture supernatant of hybrids obtained by cellular fusion (Hybridoma Technology). It is a monoclonal antibody for ABO grouping. The reagent is for in-vitro use only.

The ABO Grouping is defined both by the presence of ‘A’ and/or B’ antigens on the surface of red blood cells and by the simultaneous presence of anti-A and/or anti-B antibodies in the serum. An individual has in his serum the antibodies corresponding to the antigens which are not on his red blood cells. It is thus necessary to identify the erythrocyte antigens by the known ANTI- A, ANTI- B and ANTI- AB monoclonal antibodies(forward typing) and then confirm the results by verifying the presence of corresponding antibodies in the blood serum to be tested with control cells: ‘A1’, ‘A2‘, ‘B’ and ‘O’ (Reverse typing). Human red blood cells possessing ‘A’ and /or ‘B’ antigen will agglutinate in the presence of antibody directed towards the antigen. For example agglutination of RBCs with Anti –B monoclonal antibodies indicates the presence of ‘B’ Antigen on the red blood cells.

The Antibodies are stable at 2-8°C for 18 Months.

ORDERING INFORMATION

PRODUCT NAME	PACK SIZE	CATALOG NO.
ANTI B MONOCLONAL ANTIBODIES	10 ml	BR240010

PERFORMANCE CHARACTERISTICS

Avidity	Intensity	Titre
2-4 seconds	++++	≥256

ANTI AB MONOCLONAL ANTIBODIES

INTENDED USE

Anti-AB Monoclonal Antibodies are in-vitro culture supernatant of hybrids obtained by cellular fusion (Hybridoma Technology). It is a monoclonal antibody for ABO grouping. The reagent is for in-vitro use only.

The ABO Grouping is defined both by the presence of ‘A’ and/or B’ antigens on the surface of the red blood cells and by the simultaneous presence of anti-A and/or anti-B antibodies in the serum. An individual has in his serum the antibodies corresponding to the antigens which are not on his red blood cells. It is thus necessary to identify the erythrocyte antigens by the known ANTI-A, ANTI-B and ANTI-AB monoclonal antibodies(forward typing) and then confirm the results by verifying the presence of corresponding antibodies in the blood serum to be tested with control cells:’A1,‘A2, ‘B’ and ‘O’ (Reverse typing). Human red blood cells possessing ‘A’ and /or ‘B’ antigen will agglutinate in the presence of antibody directed towards the antigen. For example agglutination of RBCs with Anti–A as well as with Anti-B monoclonal antibodies indicates the presence of both ‘A’ & ‘B’ antigen on the red blood cells.

The Antibodies are stable at 2-8°C for 18 Months.

ORDERING INFORMATION

PRODUCT NAME	PACK SIZE	CATALOG NO.
ANTI AB MONOCLONAL ANTIBODIES	10 ml	BR250010

PERFORMANCE CHARACTERISTICS

Avidity	Intensity	Titre
2-4 seconds	++++	≥256

ANTI D (IgM) MONOCLONAL ANTIBODIES

INTENDED USE

Anti-D (IgM) Monoclonal Antibodies are in-vitro culture supernatant of hybrids obtained by cellular fusion (Hybridoma Technology). It is a monoclonal antibody for Rh typing and is for in-vitro use only.

Human red cells are classified as Rh positive (Rh +) or Rh negative (Rh -) depending upon the presence or absence of ‘D’ antigen on them. Major percentage of the population is Rh positive. Human red blood cells possessing D antigen will agglutinate in the presence of corresponding antibody. Agglutination of red cells with Anti-D (IgM) Monoclonal antibodies indicates the presence of D-antigen and hence Rh positive result. It can detect D antigen (Rh1) & high grade Du antigen (weaker variant of antigen D). Incase of no agglutination is obtained with Anti- D (IgM) Monoclonal antibodies, the cells should be then checked for the presence of low grade Du antigen by Coomb’s test using Anti-D (IgG) & Anti Human Globulin serum (Coomb’s Reagent).

The Antibodies are stable at 2-8°C for 18 Months.

ORDERING INFORMATION

PRODUCT NAME	PACK SIZE	CATALOG NO.
ANTI D (IgM) MONOCLONAL ANTIBODIES	10 ml	BR260010

PERFORMANCE CHARACTERISTICS

Avidity	Intensity	Titre
4-7 seconds	++++	≥256

ANTI D (IgG) MONOCLONAL ANTIBODIES

INTENDED USE

Anti D (IgG) Monoclonal Antibodies are in-vitro culture supernatant of hybrids obtained by cellular fusion (Hybridoma Technology). It is a monoclonal antibody for Rh typing and is for in-vitro use only. For direct and indirect antiglobulin test.

Human red cells are classified as Rh positive (Rh +) or Rh negative (Rh -) depending upon the presence or absence of ‘D’ antigen on them. Major percentage of the population is Rh positive. Human red blood cells possessing D antigen will agglutinate in the presence of corresponding antibody. The red blood cells are first checked using Anti-D (IgM) monoclonal antibodies. Agglutination of red cells with Anti- D (IgM) monoclonal antibodies indicates the presence of D-antigen and hence Rh positive result. If no agglutination is obtained then the red blood cells are checked with Anti-D (IgG) monoclonal antibodies as it sensitize the Rh positive/ Du positive cells which will give agglutination on addition of coomb’s reagent (Indirect coomb’s technique).

The Antibodies are stable at 2-8°C for 18 Months.

ORDERING INFORMATION

PRODUCT NAME	PACK SIZE	CATALOG NO.
ANTI D (IgG) MONOCLONAL ANTIBODIES	10 ml	BR270010

ANTI D (IgM +IgG) MONOCLONAL ANTIBODIES

INTENDED USE

Anti-D (IgM + IgG) Monoclonal Antibodies are in-vitro culture supernatant of hybrids obtained by cellular fusion (Hybridoma technology). The Anti-D (IgM+IgG) monoclonal antibodies have the following features:

It agglutinates in saline solution.
Active at room temperature and 37°C.
Usable on glass slide as well as in the tube.

Human red cells are classified as Rh positive (Rh+ ) or Rh negative (Rh- ) depending upon the presence or absence of “D” antigen on them. Major percentage of the population is Rh positive. Human red blood cells possessing D antigen will agglutinate in the presence of corresponding antibody. Agglutination of red cells with Anti-D (IgM + IgG) Monoclonal Antibodies indicates the presence of D-antigen and hence Rh positive result. Since the antibodies is a mixture of immunoglobulin class Monoclonal IgM and Monoclonal IgG. It can detect D antigen & higher and lower grade of Du antigen i.e. the weak variant of Antigen ‘D’. In case no agglutination is obtained with Anti-D (IgM + IgG) Monoclonal Antibodies on slide, the red cells should be further checked for the presence of DU antigen using Anti Human Globulin serum (Coomb’s Reagent) by Indirect Coomb’s technique.

The Antibodies are stable at 2-8°C for 18 Months.

ORDERING INFORMATION

PRODUCT NAME	PACK SIZE	CATALOG NO.
ANTI D (IgM + IgG) MONOCLONAL ANTIBODIES	10 ml	BR280010

BOVINE ALBUMIN

INTENDED USE

Bovine Albumin is primarily used to enhance the reactivity of blood grouping antibodies, either in direct agglutination tests or indirect anti-globulin test which can be qualitatively used in antibody detection, identification, titration and control of Rh typing.

Bovine albumin is frequently used as a control for Rh typing. Every blood specimen tested by the slide, stick or modified tube method should be controlled by testing simultaneously with a medium such as Bovine Albumin. Since cells coated with an auto-agglutinin or suspended in serum having a protein abnormality may give a false positive result in Rh typing.

The Antibodies are stable at 2-8°C for 24 Months.

ORDERING INFORMATION

PRODUCT NAME	PACK SIZE	CATALOG NO.
BOVINE ALBUMIN	5 ml 10 ml	BR070005 BR070010

(COOMB’s ANTISERA) ANTI-HUMAN SERUM

INTENDED USE

Anti-human serum (COOMB”S Antisera) is an important diagnostic aid in determining the presence or absence of red blood cell antibody or components of human complement on red blood cells. Accordingly anti-human serum is used for compatibility testing, antibody detection, antibody identification, testing for the variant of the Rho (D) antigen (DU tests), and umbilical cord red blood cell testing. Anti-human serum may be used in the direct antiglobulin test and in the indirect antiglobulin test to detect antibodies and/or complement on red blood cells.

The procedures used with the reagent are based on the principle of heteroagglutinins directed against components of human serum as originally described by Moreschin and agglutination as described by Landsteiner.Normal human red blood cells, in the presence of antibody directed toward an antigen they possess, may become sensitize but fail to agglutinate due to the particular nature of the antigen and antibody involved. Anti-human serum will react with red cells sensitized with gamma globulin (red blood cell antibody) or components of human complement and cause agglutination of the red blood cells.

COOMB’S Antisera (Anti-human serum) is stable at 2-8°C for 12 months

ORDERING INFORMATION

PRODUCT NAME	PACK SIZE	CATALOG NO.
(COOMB’s ANTISERA) Anti-human serum	5 ml 10 ml	BR050005 BR050010

ANTI-A1 LECTIN

INTENDED USE

Erythrocytes possessing the A antigen can be sub divided into A1 and A2 cells. Anti- A1 Lectin is designed for use in agglutination tests for the detection of the A1 antigen on human red cells. Group A red blood cells which are agglutinated with Anti- A1 Lectin are said to be of sub-group A1.Those which are not agglutinated by Anti-A1 Lectin fall into subgroups weaker than A1, the majority being classified as A2. Approximately 80 % of the population of blood group A is A1, while the remaining 20% are A2 or weaker subgroup.

The procedure used with this reagent is based on the principle of agglutination described by Landsteiner. Normal human red blood cells possessing antigens will clump in the presence of antibody directed towards the antigens. Renkonen showed that some extracts of seeds (lectin) contain powerful agglutinins with human blood group specificity. The extract of the seeds from Dolichos biflorus is virtually specific for A1 antigen on red blood cells.

Anti A1 Lectin is stable at 2-8°C for 24 months.

ORDERING INFORMATION

PRODUCT NAME	PACK SIZE	CATALOG NO.
ANTI-A1 LECTIN	5 ml	TB060005

ANTI-H LECTIN

INTENDED USE

The Anti-H Lectin is a purified extract from seeds of Ulex europaeus. The reagent contains a phytohaemagglutinin which is virtually specific for the H antigen on human red blood cells. Anti-H Lectin is used to demonstrate the presence of H antigen on human red blood cells and in assessing the H secretor status of group ‘O’ individuals.

Major percentage of the population (80%) has soluble ABO blood group antigens present in their body fluids. These patients are known as ABH secretors while remaining 20% are non secretors. Secretors contain the following ABH antigens in their body fluids:

O Secretors : Only H : A Secretors : A & H
B Secretors : B & H : AB Secretors : A,B & H
Non Secretors : None

Human red blood cells possessing H antigen will agglutinate in the presence of seed extract of Ulex europaeus containing phytoagglutinin specifically directed towards it. Saliva also contains water soluble ‘H’ antigen which gets neutralized by Anti-H Lectin. Agglutination of red cells/neutralization of Anti-H Lectin by saliva is a positive test result and indicates the presence of H substance on/in the red cells/saliva respectively. No agglutination of red cells/neutralization of Anti – H Lectin by saliva is a negative test result and indicates the absence of H substance on/in the red cells/saliva respectively.

Anti H Lectin is stable at 2-8°C for 24 months.

ORDERING INFORMATION

PRODUCT NAME	PACK SIZE	CATALOG NO.
Anti-H Lectin	5 ml	BR080005

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